National Pingtung University of Science and Technology / Associate Professor Hsiang-Yu Wang
Pain Points Solved
This research developed an antioxidant culture method and its auxiliary equipment, aiming to solve the problems of increased oxidative stress, cell senescence, and reduced production efficiency under high-density cell culture. Through electrolytic hydrogen technology and a 3D-printed peristaltic pump system, it effectively reduces intracellular reactive oxygen species (ROS), enhancing cell activity and viral antigen yield. This technology has been verified for its efficacy in Bovine Ephemeral Fever Virus (BEFV) vaccine production and can be applied to various virus and stem cell culture fields, possessing extensive industrial application potential.
Technology Introduction
This equipment can be applied to various types of bioreactors to reduce oxidative stress and cell aging in high-density cultures, and possesses high biological safety with no drug residues or toxic by-products. It can enhance cell susceptibility to viruses, accelerate production cycles, and increase cell activity and density. The overall process is more efficient, can lower costs and reduce carbon emissions, possessing potential for industrial application.
Figure 1. Senescent cell state induced under high-density cell culture stress.
Figure 2. Mechanism of hydrogen molecules on anti-oxidation, anti-inflammation, and cytoprotection.
Application Examples
Verification Case 1: Efficacy verification of antioxidant auxiliary novel equipment applied to BEFV vaccine production manufacturing.
Verification Case 2: Application technology of dynamic cell culture antioxidant in vitro model in fully automated 2D/3D dynamic culture system
Related Links
None
Patent Name and Number
I840889
Industry-Academia / Tech Transfer Partner
None
Honors and Awards
Taiwan Innotech Expo Invention Competition
Technical Contact
Joan Li, Project Manager
National Pingtung University of Science and Technology
Tel: +886 8-7703202 ext. 6571
Email: joanli@mail.npust.edu.tw