Technology Introduction
KTU is a precision identification platform for microorganisms and probiotics, built on DNA k-mer frequency features and machine learning. For amplicon sequence variants (ASVs) generated by 16S rRNA sequencing, this technology further re-clusters sequences while preserving the advantages of high resolution and error correction. At the same time, it reduces the overestimation of alpha diversity and the dispersion of biological signals caused by intraspecies variation. By constructing microbial sequence fingerprints based on k-mer frequencies and integrating K-medoids clustering, KTU identifies microbial analytical units with greater biological and taxonomic relevance. This platform can be applied to gut microbiota analysis, evaluation of probiotic intervention efficacy, strain typing, product quality control, and association analyses with clinical or environmental parameters, helping probiotic and microbiome-related products establish more precise and credible scientific evidence.
Figure 1. KTU 的演算法核心
Figure 2. 透過KTU鑑定酸種老麵中的微生物，精確類聚同功(function)、同屬(genus)乳酸菌的DNA序列
Figure 3. 透過KTU分析微生物發酵槽，可以清楚解析菌種組成動態隨時間的變化趨勢 （比較g圖有KTU處理和h圖沒有KTU處理）
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Technical Contact
Industrial Liaison Office
National Sun Yat-sen University
Tel: +886 7-5250165
Email: gloria@mail.nsysu.edu.tw