National Pingtung University of Science and Technology /  Associate Professor Hsiang-Yu Wang

 Pain Points Solved 

This research developed an antioxidant culture method and its auxiliary equipment, aiming to solve the problems of increased oxidative stress, cell senescence, and reduced production efficiency under high-density cell culture. Through electrolytic hydrogen technology and a 3D-printed peristaltic pump system, it effectively reduces intracellular reactive oxygen species (ROS), enhancing cell activity and viral antigen yield. This technology has been verified for its efficacy in Bovine Ephemeral Fever Virus (BEFV) vaccine production and can be applied to various virus and stem cell culture fields, possessing extensive industrial application potential.

 Technology Introduction 

This equipment can be applied to various types of bioreactors to reduce oxidative stress and cell aging in high-density cultures, and possesses high biological safety with no drug residues or toxic by-products. It can enhance cell susceptibility to viruses, accelerate production cycles, and increase cell activity and density. The overall process is more efficient, can lower costs and reduce carbon emissions, possessing potential for industrial application.

Figure 1. Senescent cell state induced under high-density cell culture stress.

Figure 2. Mechanism of hydrogen molecules on anti-oxidation, anti-inflammation, and cytoprotection.

 Application Examples 

Verification Case 1: Efficacy verification of antioxidant auxiliary novel equipment applied to BEFV vaccine production manufacturing. 

Verification Case 2: Application technology of dynamic cell culture antioxidant in vitro model in fully automated 2D/3D dynamic culture system

 Related Links 

None

 Patent Name and Number 

I840889

 Industry-Academia / Tech Transfer Partner 

None

 Honors and Awards  

Taiwan Innotech Expo Invention Competition 

 Technical Contact  

Joan Li, Project Manager

National Pingtung University of Science and Technology
Tel: +886 8-7703202 ext. 6571
Email: joanli@mail.npust.edu.tw